For laboratory & research use only · Worldwide cold-chain shipping
Quality & Testing

Reading an HPLC purity report: a short primer

High-performance liquid chromatography is the workhorse method for assessing the purity of reference materials. Here is a brief primer on interpreting a report.

The chromatogram

Each peak represents a component eluting from the column at a characteristic retention time. The main peak corresponds to the target compound.

Area percent

Purity is typically reported as the area of the main peak divided by the total peak area, expressed as a percentage.

Why method matters

Column chemistry, gradient and detection wavelength all influence the result, which is why methods are reported alongside the figure.

Leave a comment

您的邮箱地址不会被公开。 必填项已用 * 标注